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Molecular Weight of Soy Protein Isolate (SPI) after Successive Purification Steps

Prof. Rakesh Kumar


Soy protein isolate (SPI) was dissolved in 0.1 M NaOH and 0.1 M NaCl. Several purification steps were carried out so as to remove most of the insoluble’s present in SPI. Dilute solution behavior of SPI in 0.1 M NaOH and 0.1 M NaCl was examined by laser light scattering and Ubbelohde capillary viscometer. The dependence of molecular weight on purification steps was examined and reported. This work provided a novel and simple solvent system that can be used to investigate the molecular weight of SPI. 


SPI, viscosity, laser light scattering, molecular weight

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